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The cDNA was amplified with SYBR QPCR mixture (Yeasen Bio, Shanghai, China) on the BIO-RAD CFX Connect Real-time System (BioRad, CA, United States)

Yu J, Chai P, Xie M, Ge S, Ruan J, Fan X, et al

Heat stress has been shown to activate apoptotic cascades, inducing alterations in the oocyte phospholipid membrane 16 and ultimately promoting detrimental effects on oocyte developmental competence 50

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