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Quantification of phosphorylated Jak2 and tubulin expressions were performed by calculating the band density using 1D Digital Analysis Software (Kodak Digital ScienceTM, Eastman Kodak Company, Rochester, NY, USA)

Evans blue extravasation refers to the potential leakage of Evans blue dye from blood vessels into the surrounding tissues, which potentially indicates increased vascular permeability, possibly associated with inflammation

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