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CyTOF Metal-isotope conjugated monoclonal antibodies (Supplementary Data 2) targeting T cell phenotypic, activation, and metabolic proteins were used to evaluate the metabolic and activation properties of patient-derived CAR T cells in vitro and ex vivo

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After adding 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) to a final concentration of 10 mM and setting the pH to 6.2 using 5 N NaOH, the product was extracted four times with 1 vol of ethyl acetate

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