Proteins were then precipitated by the addition of the total sample volume to 4 volume liquid chromatographymass spectrometry (LCMS)-grade acetonitrile, as per the SP4 protocol 63 , followed by gentle shaking for 5 min and centrifugation at 3,220 g for 10 min
Damilola A
Significant differential metabolites in the three comparisons were PE-NMe2 (20:2(11Z,14Z)/15:0), PC (22:2(13Z,16Z)/12:0), L-Alloisoleucine, PS (O-18:0/13:0), Photinus Luciferin, 5beta-Cholestan-3alpha,4alpha,11beta,12beta,21-Pentol-3,21-Disulfate, Ixocarpanolide, 2-Piperidinone, PS [(18:2(9Z,12Z)/22:5(4Z,7Z,10Z
improved water, sanitation and hygiene
Since the parental ECOR strains were typically quite resistant to phage infection to begin with, only mutants with transposon-inactivated defense systems would be expected to drop out upon infection