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The gold standard for measuring viability is the CellTiter-Glo Viability Assay: Single-reagent, single-step ATP detection Most-cited viability assay in peer-reviewed research Enables scaling for high-throughput screening Can I track viability in real time

Supernatants were collected into fresh tubes and GSH and GSSG levels were assessed by the GSR-DTNB recycling assay as described in Gherghel D, et al

Unlike chronic neurodegenerative diseases such as PD, AD, HD or ALS, which involve slowly progressive and multifactorial neuronal loss, stroke represents an acute insult where the timing of intervention is critical