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Metabolite profiling of reduced thiol-containing metabolites was carried out in the presence of NEM

Cell culture, treatment, and transfection Human HEK293T and HT1080 cells were obtained from the American Type Culture Collection (ATCC) and maintained (37 C, 5% CO 2 ) in DMEM high-glucose medium (Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), penicillinstreptomycin (100 U/mL, Gibco, 15140148), sodium pyruvate (1 mM, Gibco, 11360070), and MEM nonessential amino acids (1, Gibco, 11140050)

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The rapid CHOP activation indicated that cytotoxicity of AQ-QI and DEAQ-QI was accompanied by the activation of the adaptive ER stress response pathway and subsequent activation of apoptotic programs (Tew and Townsend, 2012

Ramazani Z, Nakhaee S, Sharafi K, Rezaei Z, Mansouri B (2024) Autism spectrum disorder: cadmium and mercury concentrations in different biological samples, a systematic literature review and meta-analysis of human studies