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doi: 10.1016/j.redox.2024.103205 [DOI] [PMC free article] [PubMed] [Google Scholar] 103.Forsythe JA, Jiang BH, Iyer NV, et al

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The test is usually terminated once the patient experiences a 20% decrease in breathing ability, or a maximum test dose of methacholine is achieved (American Lung Association - Methacholine Challenge Test)