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Land was cheap and means of fertilization was limited and laborious

Cells were trypsinized, pelleted, and washed with ice-cold phosphate-buffered saline (PBS) followed by resuspension in radioimmunoprecipitation assay (RIPA) buffer supplemented with 1X Protein Inhibitor Cocktail and phenylmethylsulfonyl fluoride (PMSF) (Roche)

For the year through May, the level was steady at a 0.1% increase

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