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The results indicate that Nrf2 positively regulates SLC7A11, TXNRD1, GSS, GCL, GSR, GPX4, FSP1, PPP enzymes, and HO-1, enhancing antioxidant capacity and inhibiting ferroptosis (Figure 1D)
Nature 475 , 2730 (2011)
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Analysis of single-cell RNA sequencing Single-cell RNA sequencing (scRNA-seq) data (HRA000425) were downloaded from Genome Sequence Archive (SRA)-Human databases in China National Center for Bioinformation 16 , converted into FASTQ format using fasterq-dump software (sratoolkit 3.0.2, NCBI, NIH, Bethesda, MD, USA), converted into a matrix data file using Cell Ranger software version 3.0.2 (10xGenomics, Pleasanton, CA, USA), converted into Seurat objects using Seurat 5.0.1 software (Satija lab, Lower Manhattan, New York, NY, USA), and plotted for t -distributed stochastic neighbor embedding ( t -SNE) visualization
