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Unsmoked cigarette filters (without any tobacco remnants) were also found to be toxic, with LC50 values of 5.1 and 13.5 cigarette butts/L, respectively for both fish species
Additionally, while our study predominantly examined prevalent additives in our study population, such as slaked lime and tobacco, future research could broaden the scope by assessing the carcinogenic potential of additives used in other countries
Based on proteomics data, concentration-response experiments were conducted using BEAS-2B bronchial epithelial cells to evaluate reactive oxygen species, mitochondrial function, and cytoskeletal integrity