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The regression equation was depicted as: F F 0 = 238.6 l g C + 2440.1, the corresponding correlation coefficient ( R 2 ) of calibration curve was 0.997 and the detection limit of GSH detection was calculated to be 2.0 10 11 M (3)

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GHK-Cu Research Peptide Mechanistic Profile GHKCus biological activity is defined by its copperbinding properties and its involvement in ECM and redoxassociated pathways: Copper Coordination & Delivery GHK binds Cu with high affinity Supports studies of copperdependent enzymatic systems Enables investigation of metalion transport and metalloprotein interactions GeneExpression Signaling Used in transcriptomic models examining ECMassociated and redoxlinked gene networks Supports research into pathways related to cellularstress responses ECMAssociated Biology Frequently used in studies involving fibroblastsignaling pathways Enables examination of integrinassociated and matrixregulation mechanisms Redox & Metalloprotein Activity Supports research into copperdependent enzymes such as superoxide dismutase and lysyl oxidase Useful for studying oxidativebalance pathways and metalregulated cellular responses These mechanistic domains position GHKCu as a versatile tool for studying metallopeptide biology , ECMassociated signaling , and oxidativestress response pathways