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Absorbance at 430 nm was recorded with a microplate reader (FLUOstar, BMG) at room temperature

Similarly, in an earlier study, the up-regulation of iNOS in the intestinal epithelial cells (IECs) has been closely associated with the initiation and maintenance of intestinal inflammation in IBD, which can be potentially used as a non-invasive blood-based biomarker of IBD, as documented (139)