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doi:10.1002/dta.1715
Never shake vigorously, since mechanical agitation can fragment sensitive peptide chains and reduce the reproducibility of subsequent measurements
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Here's a comprehensive list: Chemical Reagents Fmoc-protected amino acids Coupling reagents (e.g., HBTU, HATU, or DIC) Solvents (e.g., DMF, DCM, NMP) Deprotection reagents (e.g., piperidine) Cleavage cocktail components (e.g., TFA, water, triisopropylsilane) Equipment Peptide synthesizer (automated or manual) HPLC system for purification Mass spectrometer for peptide characterization Lyophilizer for freeze-drying Rotary evaporator Analytical balance Consumables Solid support resin (e.g., Wang resin) Reaction vessels Filtration apparatus HPLC columns Having these materials on hand is crucial for the successful preparation of BPC 157 powder
The commonly used macro structural measurements (determination of the mechanical behavior, dissolution testing) [7]
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