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Although the time course of Nrf2 stabilization and nuclear translocation indicated a rapid increase with a peak at approximately 6 h for DEM, and a slow but gradual rise triggered by NFT, maximal accumulation of Nrf2 protein, as well as the induction of GCLC and glutathione synthesis, were almost identical for both NFT and DEM after incubation periods of and beyond 24 h (Fig

M., Xu S

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Samples were immediately returned to the fume cupboard and evaporated under air at 45C until dry and re-dissolved in 100 ml of 70% (v/v) acetonitrile in water prior to analysis by ESI -MS/MS