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The cells were incubated overnight at 4 C

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As a global genetic root cause consultant, Dan Purser MD has treated thousands of patients worldwidefrom the US East Coast to Dubai, Norway, and the UKwho've struggled with these symptoms

The protein concentration was quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) per the manufacturers instruction

The study protocol was approved by the ethics committee of Louisiana State Health Sciences Center-Animal Care Committee (Animal Care protocol number P-15-006), and all the methods were carried out following the approved guidelines