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After a 12-min incubation at 25 C, the temperature was elevated to 37 C and maintained for 30 min, to promote macrocyclization

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Thus, as shown in Figure 16 , the (probably not surprising) conclusion is that niclosamide can readily revert to its most stable solid forms (--the monohydrates and similar cosolvates identified by van Tonder et al (2, 62)) that have a lower solubility of niclosamide in aqueous solution when in contact with these solid forms