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As for any metabolite assay, it is advisable to check extraction efficiency, for example, with recovery experiments using known amounts of GSH or GSSG added at the stage of extraction in the presence or absence of the tissue under examination

A smaller quantity may lead to frequent reorders, while a larger quantity may not be suitable if the plan is still being adjusted

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Both NAPQI and ROS cause mitochondrial DNA damage, activation of the JNK signaling pathway, which further amplifies the mitochondrial ROS production, and causes the mitochondrial permeability transition (MPT) pore to open (Iorga et al., 2017)

Withdraw the needle at the same angle you inserted it