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The blood samples were centrifuged at 5000 g for 10 min at 4C to collect plasma, which was then stored at 20C until analysis

Diurnal rhythm of plasma delta-sleep-inducing peptide in humans: correlation with body temperature and sleep stages

shorter durations of use may also be protective

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It is known that amino acid conjugation of exogenous carboxylic acids occurs in a twostep process (Reilly et al., 2007)