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The number of samples was determined by the Universal Population Volume Known Sampling Calculation Formula (n = Nz 2 pq/d 2 (N 1) + z 2 pq)

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It requires: verifying the chemical compatibility between the peptides (cross-stability), optimizing the ratio to maintain the bioactivity of each component, individual quality control of each peptide before co-formulation, and validating the stability of the lyophilized mixture over time

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