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Introduction Busulfan is a bifunctional alkylating agent commonly used in conditioning regimens prior to hematopoietic cell transplantation (HCT) for the treatment of a variety of childhood diseases, including both malignant and non-malignant disorders

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Tissue was then transferred to 5 ml Gentle Cell Dissociation Reagent (Stemcell Technologies) and incubated at 4 C on a rocking platform for 30 min

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PMCID: PMC7053614