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(3) Bruno BJ, Miller GD, Lim CS

Then cells were washed three times with PBS followed by incubation with the secondary antibody (1:200, Proteintech) and Hoechst 33342 (1:1000) for 1 h

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Further work using ribose treatment has shown that AGE crosslinking negatively affects the ability of collagen fibrils to respond to loading after the yield point and results in a mechanically inferior tissue (Lee and Veres, 2019)