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For western blot-based competitive ABPP with RSL3-alkyne or SCLA2a samples were washed in 500-l solutions of: 1x 500 l 1% SDS in 50 mM HEPES and 2x 500 l 0.2% SDS in 50 mM HEPES

Daily doses for extended periods (months to years) push into territory where chronic effects could emerge without warning

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