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Simulation system equilibration and energy minimization Following preparation, the systems were energy minimized with an energy tolerance of 100 kj/mol and equilibrated in six sequential steps involving loosening of positional restraints on lipid atoms, protein backbone atoms, and protein side chain atoms (equilibration parameters in Extended Data Table 2)

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Int J Mol Sci 25(8):4463 Kahaly GJ (2020) Management of Graves thyroidal and extrathyroidal disease: an update