After incubation, the stain was removed and monolayers were washed, at least 3 times, with PBS (until the PBS was clear)
doi: 10.1152/physiolgenomics.00081.2016 5 AlbillosA.de GottardiA.RescignoM
However, you should: Call 999 or go to A&E immediately if you experience signs of a severe allergic reaction (difficulty breathing, severe throat/facial swelling, or collapse) Contact your GP if you experience a rash, itching, persistent pain at the injection site, or worsening neurological symptoms despite treatment If you suspect any side effects from your B12 injections, you can report them through the MHRA Yellow Card scheme (yellowcard.mhra.gov.uk)
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