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The most intense peaks (1 and 5) were subjected to MS2 analysis and both yielded identical fragments ions of m/z 261.1, 231.0 and 203.1

doi: 10.1002/mds.28206 120 MondelloS.ConstantinescuR.ZetterbergH.AndreassonU.HolmbergB.JerominA

Then there's acetic acid, which is sometimes required for peptides that are difficult to dissolve or need a specific pH to remain stable

In REDEFINE 1, Novo Nordisk reported a 22.7% mean weight reduction under the trial-product estimand for CagriSema in adults with overweight or obesity

For protein expression, a 1:100 dilution of the preculture was done in 2xTY (16 g tryptone, 10 g yeast extract, 5 g NaCl per liter)