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A significant percentage of the ME/CFS population possesses mutations in the MTHFR (methylenetetrahydrofolate reductase) gene

The cells were maintained in RPMI/B27 plus CHIR99021 for the first 24 hours, followed by its removal and re-feeding with just RPMI/B27 (with or without insulin) and maintained for a further 24 hours

doi: 10.1007/s11064-016-1994-x

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Cells were then resuspended in 500 L of free HBSS, strained through a 40 m cell strainer (BD Falcon), and analyzed using a flow cytometer (BD LSRFortessa Cell Analyzer, BD Biosciences) equipped with 488 nm laser for excitation