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The membranes were blocked with 5% skimmed milk for 1 h at room temperature and then incubated with the desired primary antibodies and appropriate horseradish peroxidaseconjugated (HRP) secondary antibodies

Evaluation the effect of phytosterol fraction of Chenopodium murale in comparison with Tacrolimus on mice induced atopic dermatitis

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10.1016/s0360-3016(99)00109-1 50 RamachandranK.SpeerC.NathansonL.ClarosM.SingalR

Plasma concentrations of C and S (from samples taken 24 h after last dosing) were quantified by liquid chromatographymass spectrometry (Supplementary Methods)