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Thirty or forty micrograms of proteins were loaded into each well and fractionated on 510% precast SDS-PAGE gels (Byeotime, Shanghai, China) and then transferred to a PVDF membrane (Merck Millipore, Darmstadt, Germany)

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Erastin, as a small-molecule ferroptosis inducer, works by inhibiting the system Xc transporter-an amino acid antiporter responsible for exchanging extracellular cystine for intracellular glutamate (11)-leading to depletion of intracellular glutathione (GSH), indirectly inactivating glutathione peroxidase 4 (GPX4), and ultimately triggering ferroptosis (12)

To maintain a moderate weight, also try to eat more healthfully and engage in regular exercise.

Also, the final decision to inject Neurobion is made by your doctor