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Normal human primary hepatocytes (ThermoFisher Scientific) were thawed in the Cryopreserved Hepatocyte Recovery Medium, centrifuged, then resuspended and plated in the plating medium (Williams Medium E without phenol red supplemented with Hepatocyte Plating Supplement Pack)

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Figure 1A clearly shows that the control samplethe diluted grape juice without modification (C)initially consumed oxygen very quickly

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Consistent use over several weeks to months gives the best chance of visible improvement