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Stable lines were selected with puromycin and validated by fluorescence observation and by confirming PRKN expression at mRNA and protein levels

Included populations were limited to general populations of people who smoke, those motivated or not motivated, and people with mental illness

Challenges remain, including navigating privacy regulations, ensuring device reliability to prevent false alarms, and managing the upfront investment costs for large-scale deployments

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E-cigarettes vaporize a liquid mix of glycerin, propylene glycol, nicotine, and flavorings