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Quantitative real-time PCR (qRT-PCR) and Western blot assay Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturers instructions protocol

[8] A single injection raised IGF-1 for a week or more

On a neurochemical level, NAC enhanced dopamine turnover in the SN and increased dopamine levels in the NAc of ethanol-treated animals

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