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14 NAD + biosynthesis De novo pathway Mammalian cells can generate NAD + de novo from dietary tryptophan (Trp) by the kynurenine pathway (KP), which is initialized by either TDO or IDO

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The plasmids containing the gene of pCNFRS, pET21a-sfGFP and pET21a-sfGFP2TAG was extracted by SanPrep Column Plasmid Mini-Preps Kit

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