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Immunoprecipitation and western blotting Cells were lysed with RIPA buffer, and 500 g whole cell lysate was precipitated with 2 g agarose-conjugated antibody against Flag (Flag beads) and normal IgG (as a negative control)

Pharmacological modulation of excitotoxicity through the combined use of NMDA receptor inhibition and Group III mGlu activation reduces TMT-induced neurodegeneration in the rat hippocampus

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Aliment Pharmacol Ther 2001