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For sequencing, the V4V5 region of the 16S rRNA gene was amplified from the extracted DNA using com1 (CAGCAGCCGCGGTAATAC) and com2 (CCGTCAATTCCTTTGAGTTT) primers (Lane et al., 1985), as previously described (Macey et al., 2023)

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Because of this interaction, the compound has become a subject of interest in research focused on metabolic biology and enzymatic regulation