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Growth of this strain containing the gene circuit in the presence of an inducer activates transcription of the reporter gene, producing a detectable signal, as long as the inducer exceed a specified concentration, which defines the limit of detection (LOD) or sensitivity of the circuit

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5 mg = 5,000 mcg 5,000 mcg / 2 mL = 2,500 mcg/mL 250 mcg / 2,500 mcg per mL = 0.1 mL = 10 U-100 units BPC-157 10 mg vial with 2 mL example A 10 mg BPC-157 vial contains 10,000 mcg