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The Sp-flies, in-spite of possessing increased mitochondrial content, demonstrated reduced production of mitochondrial superoxides (Fig
D., Vitonis, A
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To lessen aberrant cell accumulation and stop tumors from forming, it, on the one hand, controls downstream signals, aids in the repair of DNA damage, stops the cell cycle, and transduces the caspase signal through the activation of the mitochondrial pathway or death receptor pathway to promote apoptosis when damage cannot be repaired (159)
Western Blot Total protein content of cell lines or the tumor was extracted by lysis in radioimmunoprecipitation assay buffer containing protease inhibitor cocktail and the protein phosphatase inhibitor, and the total protein concentration was quantified by the BCA (bicinchoninic acid) method
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