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Better safe than sorry

On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

Both vials contain identical compound and purity

Future research recommendations Most of the research and clinical studies conducted on ME/CFS have inconsistent outcomes and use different study parameters, making the results difficult to compare or combine (581, 582)

That's particularly valuable for a supplement like astaxanthin, where individual responses can vary, and benefits may take several weeks to become noticeable