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Imaging was performed using an SP8 Leica confocal microscope with a 40 objective lens with 1 digital zoom, optimal pinhole and line average, bidirectional scan, speed 400600 Hz, resolution 1,024 1,024

Dox treatment induced robust expression of full-length (FL) p21 in both Control and p53 KO cells, which was not further augmented by co-treatment with nutlin-3 (Fig 2A)

About Glutathione peptide Glutathione acts as a major intracellular antioxidant and redox buffer, primarily through the reactive sulfur group in its cysteine residue

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