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The sorted cells were washed twice with 50 mL phosphate-buffered saline to remove the FreSHtracer and then further cultivated in culture medium for 24 h prior to in vitro or in vivo experiments, as described below

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Microglia priming by interleukin-6 signaling is enhanced in aged mice

Keep a Behavior Log : Documenting your childs baseline behavior, communication, and physical symptoms can help track changes over time