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Most cycles last 2 to 4 weeks , followed by a short break

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The freeze-thaw process is brutal on peptide chains, causing them to fracture and become useless

The entrance of the active cavity is composed of His85, Met280, Val283 and other residues, and its steric hindrance and - stacking (such as Phe264 and benzyl ring of the substrate) jointly determine the substrate selectivity (Yu and Fan, 2021)

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