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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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In hydrolysis step, Epoxide Hydrolase and Carboxylesterases use water to cleave chemical bonds, converting reactive epoxides into stable diols or breaking down esters and amides into their parent acids and alcohols (Schlenk et al., 2008)