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In human retinal pigment epithelium cells, bilberry anthocyanins have been demonstrated to modulate the oxidative stress-protective enzymes glutathione S-transferase-pi and heme oxygenase-1 (HO-1) [269]

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doi: 10.1074/jbc.M112.440214

Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h