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To purify K29/K48-Ub8, we mixed the reaction solution with four volumes of 50 mM ammonium acetate buffer (pH 4.5) and loaded them onto a Resource S cation exchange column pre-equilibrated with the same buffer

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High serum B12 is not necessarily a condition in itself, but rather an indication of an underlying condition that is affecting the transport and distribution system of B12 for cellular use

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Most of these are well tolerated and widely available, making them easy to incorporate into your daily routine