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It serves as one of the most fundamental and powerful antioxidants naturally found in the body's cells

[DOI] [PubMed] [Google Scholar] 354.Brasky TM, White E & Chen CL Long-term, supplemental, one-carbon metabolism-related vitamin B use in relation to lung cancer risk in the vitamins and lifestyle (VITAL) cohort

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The homogenate was centrifuged at 12,000 rpm for 15 min at 4C and the supernatant was collected for assay MPO activity as determined by measuring the H 2 O 2 -dependent oxidation of o -dianisidine solution (3,3-dimethoxybenzidine dihydrochloride in potassium phosphate buffer, pH 6.0) at 450 nm using an Epoch microplate spectrophotometer (BioTek, VT, USA)