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The qPCR reactions were conducted utilizing RealQ PCR Master Mix (A320799 Ampliqon, Denmark) in Rotor-Gene 6000 Real-Time Thermal Cycler (Corbett Research, Australia)

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Compounds in the samples analyzed in 2024 were identified using a liquid chromatograph with quadrupole TOF mass spectrometer (LCMS9050-Q-TOF, Shimadzu Corporation, Kyoto, Japan)

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The mechanistic and preclinical evidence sits with the individual peptides chiefly GHK-Cu, which has the deepest literature while the specific three-peptide combination is used in research settings without combination-level trial data

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