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Safranin O Staining Pellets were fixed overnight at 4C in 10% buffered formalin (Fisher Chemical, Hampton, NH, United States), dehydrated in gradient ethanol, cleared in xylene, and then embedded in paraffin

Thymosin Beta-4, Fragment (TB-500) A synthetic fragment of the naturally occurring protein Thymosin Beta-4

Phase II Detoxification: It is a key substrate for the enzyme Glutathione S-transferase (GST), which binds to toxins and xenobiotics (foreign chemical substances), rendering them water-soluble for excretion

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For total protein extraction, tissues or cells were homogenized in ice-cold RIPA lysis buffer (containing 1% protease and phosphatase inhibitors) using a mechanical homogenizer