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Our group and others have demonstrated the involvement and importance of Rho-mediated cytoskeletal rearrangements in endothelial-leukocyte interaction, and blockade of GTPase activity can prevent associated loss of Occludin and Claudin 5 localization (Persidsky et al., 2006

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That's what I what I started people wear glasses, who didn't typically wear glasses that kind of tipped me off that something was going wrong