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Nat Med 10 , 895896 (2004)

The LC-MS analysis employed state-of-the-art equipment, including an UHPLC (1290 Infinity LC, Agilent Technologies) coupled with a quadrupole time-of-flight (AB Sciex TripleTOF 6600) at Shanghai Applied Protein Technology Co., Ltd.Utilizing both HILIC and RPLC separation techniques, chromatographic separation ensured robust performance in both ESI positive and negative modes

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Advanced symptoms of Parkinsons disease include: Uncontrollable shaking and tremors Reduced ability to chew and swallow Difficulty enunciating words Impaired ambulation and frequent falls Bent-over posture rinary and fecal incontinence nability to perform activities of daily living (ADL) like dressing, bathing, and eating As Parkinsons approaches its end game, palliative care and hospice with pain management are the final treatment approaches

The disadvantage is larger injection volumes, which some people find uncomfortable