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Proc Nat Acad Sci U S A

Heme, 3,3,5,5-tetramethylbenzidine, 2-methylimidazole, Zn(NO 3 ) 2 6H 2 O, superoxide dismutase, l-ascorbic acid, l-tryptophan, l-glycine, glutamic acid, mannitol, furfuryl alcohol, l-cysteine, l-arginine, glutathione, 5,5-dimethyl-1-pyrroline N -oxide, nafion solution (99%), methanol (99.9%), and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich

OsDHAR was purified by Ni-NTA affinity chromatography [5 mL HisTrap FF column (GE Healthcare, Little Chalfont, UK) on an AKTA FPLC system] and eluted with a step gradient of 250 mM imidazole

One older study documented intravenous glutathiones positive effects on symptoms such as tremors and rigidity