HEK293T cells were used as negative controls to account for the potential inhibitory effect on cell proliferation as a result of cell confluence due to the increased cell number of coculture conditions within the same well
The cells were then divided into four treatment groups: Control, ORI@Lipo, ORI@CPD-Lipo, ORI@CPD-Lipo plus CKAAKN
Methionine (25mg) is an essential amino acid critical to healthy metabolism
Selecting the appropriate quantity depends on the scale and duration of the research programme: GHK-CU 50mg is suited to smaller-scale studies, preliminary investigations, method development, and research programmes that are in the early stages of incorporating this compound into their experimental design
An equal number of sex-, age-, site of onset-, disease-duration-matched untreated patients with ALS were analysed as a control group